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anti lc3b  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti lc3b
    Anti Lc3b, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc3%CE%B1+%CE%B2/MAP+LC3%CE%B1%2F%CE%B2+Antibody/pm41886893-112-6-8
    Average 94 stars, based on 148 article reviews
    anti lc3b - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Small Interfering RNA:

    Article Title: NLRX1 Facilitates Histoplasma capsulatum -Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.
    Article Snippet: .. Small Interfering RNA (siRNA) Transfection of Macrophages Macrophages were transfected with control siRNA or siRNA pools targeting Rubicon, LC3α/β or TUFM (Santa Cruz Biotechnology) usingTransIT-TKOTransfection Reagent (Mirus Biology LCC) according to the manufacturer’s instruction. ..

    Transfection:

    Article Title: NLRX1 Facilitates Histoplasma capsulatum -Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.
    Article Snippet: .. Small Interfering RNA (siRNA) Transfection of Macrophages Macrophages were transfected with control siRNA or siRNA pools targeting Rubicon, LC3α/β or TUFM (Santa Cruz Biotechnology) usingTransIT-TKOTransfection Reagent (Mirus Biology LCC) according to the manufacturer’s instruction. ..

    Article Title: NLRX1 Facilitates Histoplasma capsulatum -Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages
    Article Snippet: .. Macrophages were transfected with control siRNA or siRNA pools targeting Rubicon, LC3α/β or TUFM (Santa Cruz Biotechnology) using Trans IT-TKO Transfection Reagent (Mirus Biology LCC) according to the manufacturer's instruction. ..

    Control:

    Article Title: NLRX1 Facilitates Histoplasma capsulatum -Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.
    Article Snippet: .. Small Interfering RNA (siRNA) Transfection of Macrophages Macrophages were transfected with control siRNA or siRNA pools targeting Rubicon, LC3α/β or TUFM (Santa Cruz Biotechnology) usingTransIT-TKOTransfection Reagent (Mirus Biology LCC) according to the manufacturer’s instruction. ..

    Article Title: Mitochondrial dynamics controls anti-tumour innate immunity by regulating CHIP-IRF1 axis stability.
    Article Snippet: .. Antibodies targeting IKBα (C-21, 1:1000), Fam73b (S12, 1:1000), p65 (C-20, 1:1000), Lamin B (C-20, 1:1000), ERK (K-23, 1:2000), phospho-ERK (E-4, 1:1000), JNK2 (C-17, 1:1000), p38 (H-147, 1:1000), IKKα (H744, 1:1000), ubiquitin (P4D1, 1:1000), p105/p50 (C-19, 1:1000), IRF1 (M-20, 1:1000), CHIP (H-231, 1:1000), HSP60 (H-1, 1:3000), TBK1 (108A429, 1:1000), IRF3 (SC-9082, 1:1000), LC3α/β (SC-292354, 1:1000), and c-Rel (sc-71, 1:1000), as well as a control rabbit IgG (sc-2027), were from Santa Cruz Biotechnology. .. Antibodies targeting phospho-DRP1 (Ser637, #3455, 1:1000), DRP1 (D6C7, 1:1000), Mitofusin-2 (D1E9, 1:1000), phospho-IκBα (Ser32, 14D4, 1:1000), phospho-JNK (Thr180/Tyr185, #9251, 1:1000), phospho-p38 (Thr180/Tyr182, 3D7, 1:1000), phospho-p105 (Ser933, 18E6, 1:1000), phospho-IKKα/β (Ser176/180, 16A6, 1:1000), phospho-TBK1 (Ser172, D52C2, 1:1000), phospho-IRF3 (Ser396, 4D4G, 1:1000), and K48-linkage-specific polyubiquitin (D9D5, 1:8000) were 10 NATURE COMMUNICATIONS | 8: 1805 |DOI: 10.1038/s41467-017-01919-0 |www.nature.com/naturecommunications purchased from Cell Signaling Technology Inc. Anti-actin (C-4, 1:10,000) was from Sigma.

    Article Title: NLRX1 Facilitates Histoplasma capsulatum -Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages
    Article Snippet: .. Macrophages were transfected with control siRNA or siRNA pools targeting Rubicon, LC3α/β or TUFM (Santa Cruz Biotechnology) using Trans IT-TKO Transfection Reagent (Mirus Biology LCC) according to the manufacturer's instruction. ..

    Article Title: Mitochondrial dynamics controls anti-tumour innate immunity by regulating CHIP-IRF1 axis stability
    Article Snippet: .. Antibodies targeting IKBα (C-21, 1:1000), Fam73b (S-12, 1:1000), p65 (C-20, 1:1000), Lamin B (C-20, 1:1000), ERK (K-23, 1:2000), phospho-ERK (E-4, 1:1000), JNK2 (C-17, 1:1000), p38 (H-147, 1:1000), IKKα (H-744, 1:1000), ubiquitin (P4D1, 1:1000), p105/p50 (C-19, 1:1000), IRF1 (M-20, 1:1000), CHIP (H-231, 1:1000), HSP60 (H-1, 1:3000), TBK1 (108A429, 1:1000), IRF3 (SC-9082, 1:1000), LC3α/β (SC-292354, 1:1000), and c-Rel (sc-71, 1:1000), as well as a control rabbit IgG (sc-2027), were from Santa Cruz Biotechnology. .. Antibodies targeting phospho-DRP1 (Ser637, #3455, 1:1000), DRP1 (D6C7, 1:1000), Mitofusin-2 (D1E9, 1:1000), phospho-IκBα (Ser32, 14D4, 1:1000), phospho-JNK (Thr180/Tyr185, #9251, 1:1000), phospho-p38 (Thr180/Tyr182, 3D7, 1:1000), phospho-p105 (Ser933, 18E6, 1:1000), phospho-IKKα/β (Ser176/180, 16A6, 1:1000), phospho-TBK1 (Ser172, D52C2, 1:1000), phospho-IRF3 (Ser396, 4D4G, 1:1000), and K48-linkage-specific polyubiquitin (D9D5, 1:8000) were purchased from Cell Signaling Technology Inc. Anti-actin (C-4, 1:10,000) was from Sigma.

    Blocking Assay:

    Article Title: Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte.
    Article Snippet: .. Subsequently, they were blocked with blocking buffer (Beyotime, P0102) at room temperature for a period of 1 h followed by incubation with FTH1 (Affinity, DF6278, 1:200), LC3α/β (Santa Cruz, sc-398822, 1:200) and NCOA4 (Santa Cruz, sc-373739, 1:100) at 4 °C overnight. .. Then, these cells were rinsed with PBST three times and then soaked with either fluorescent labeled Goat Anti-Mouse Secondary Antibody (Proteintech, RGAM002, 1:500) or Goat Anti-Rabbit Secondary Antibody (Proteintech, RGAR004, 1:500) at room temperature for a duration of 1 h. The testicular sections embedded in paraffin were immersed in hematoxylin for 2-min, followed by a 1-min water rinse.

    Article Title: Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte
    Article Snippet: .. Subsequently, they were blocked with blocking buffer (Beyotime, P0102) at room temperature for a period of 1 h followed by incubation with FTH1 (Affinity, DF6278, 1:200), LC3α/β (Santa Cruz, sc-398822, 1:200) and NCOA4 (Santa Cruz, sc-373739, 1:100) at 4 °C overnight. .. Then, these cells were rinsed with PBST three times and then soaked with either fluorescent labeled Goat Anti-Mouse Secondary Antibody (Proteintech, RGAM002, 1:500) or Goat Anti-Rabbit Secondary Antibody (Proteintech, RGAR004, 1:500) at room temperature for a duration of 1 h. The testicular sections embedded in paraffin were immersed in hematoxylin for 2-min, followed by a 1-min water rinse.

    Incubation:

    Article Title: Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte.
    Article Snippet: .. Subsequently, they were blocked with blocking buffer (Beyotime, P0102) at room temperature for a period of 1 h followed by incubation with FTH1 (Affinity, DF6278, 1:200), LC3α/β (Santa Cruz, sc-398822, 1:200) and NCOA4 (Santa Cruz, sc-373739, 1:100) at 4 °C overnight. .. Then, these cells were rinsed with PBST three times and then soaked with either fluorescent labeled Goat Anti-Mouse Secondary Antibody (Proteintech, RGAM002, 1:500) or Goat Anti-Rabbit Secondary Antibody (Proteintech, RGAR004, 1:500) at room temperature for a duration of 1 h. The testicular sections embedded in paraffin were immersed in hematoxylin for 2-min, followed by a 1-min water rinse.

    Article Title: Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte
    Article Snippet: .. Subsequently, they were blocked with blocking buffer (Beyotime, P0102) at room temperature for a period of 1 h followed by incubation with FTH1 (Affinity, DF6278, 1:200), LC3α/β (Santa Cruz, sc-398822, 1:200) and NCOA4 (Santa Cruz, sc-373739, 1:100) at 4 °C overnight. .. Then, these cells were rinsed with PBST three times and then soaked with either fluorescent labeled Goat Anti-Mouse Secondary Antibody (Proteintech, RGAM002, 1:500) or Goat Anti-Rabbit Secondary Antibody (Proteintech, RGAR004, 1:500) at room temperature for a duration of 1 h. The testicular sections embedded in paraffin were immersed in hematoxylin for 2-min, followed by a 1-min water rinse.

    Ubiquitin Proteomics:

    Article Title: Mitochondrial dynamics controls anti-tumour innate immunity by regulating CHIP-IRF1 axis stability.
    Article Snippet: .. Antibodies targeting IKBα (C-21, 1:1000), Fam73b (S12, 1:1000), p65 (C-20, 1:1000), Lamin B (C-20, 1:1000), ERK (K-23, 1:2000), phospho-ERK (E-4, 1:1000), JNK2 (C-17, 1:1000), p38 (H-147, 1:1000), IKKα (H744, 1:1000), ubiquitin (P4D1, 1:1000), p105/p50 (C-19, 1:1000), IRF1 (M-20, 1:1000), CHIP (H-231, 1:1000), HSP60 (H-1, 1:3000), TBK1 (108A429, 1:1000), IRF3 (SC-9082, 1:1000), LC3α/β (SC-292354, 1:1000), and c-Rel (sc-71, 1:1000), as well as a control rabbit IgG (sc-2027), were from Santa Cruz Biotechnology. .. Antibodies targeting phospho-DRP1 (Ser637, #3455, 1:1000), DRP1 (D6C7, 1:1000), Mitofusin-2 (D1E9, 1:1000), phospho-IκBα (Ser32, 14D4, 1:1000), phospho-JNK (Thr180/Tyr185, #9251, 1:1000), phospho-p38 (Thr180/Tyr182, 3D7, 1:1000), phospho-p105 (Ser933, 18E6, 1:1000), phospho-IKKα/β (Ser176/180, 16A6, 1:1000), phospho-TBK1 (Ser172, D52C2, 1:1000), phospho-IRF3 (Ser396, 4D4G, 1:1000), and K48-linkage-specific polyubiquitin (D9D5, 1:8000) were 10 NATURE COMMUNICATIONS | 8: 1805 |DOI: 10.1038/s41467-017-01919-0 |www.nature.com/naturecommunications purchased from Cell Signaling Technology Inc. Anti-actin (C-4, 1:10,000) was from Sigma.

    Article Title: Mitochondrial dynamics controls anti-tumour innate immunity by regulating CHIP-IRF1 axis stability
    Article Snippet: .. Antibodies targeting IKBα (C-21, 1:1000), Fam73b (S-12, 1:1000), p65 (C-20, 1:1000), Lamin B (C-20, 1:1000), ERK (K-23, 1:2000), phospho-ERK (E-4, 1:1000), JNK2 (C-17, 1:1000), p38 (H-147, 1:1000), IKKα (H-744, 1:1000), ubiquitin (P4D1, 1:1000), p105/p50 (C-19, 1:1000), IRF1 (M-20, 1:1000), CHIP (H-231, 1:1000), HSP60 (H-1, 1:3000), TBK1 (108A429, 1:1000), IRF3 (SC-9082, 1:1000), LC3α/β (SC-292354, 1:1000), and c-Rel (sc-71, 1:1000), as well as a control rabbit IgG (sc-2027), were from Santa Cruz Biotechnology. .. Antibodies targeting phospho-DRP1 (Ser637, #3455, 1:1000), DRP1 (D6C7, 1:1000), Mitofusin-2 (D1E9, 1:1000), phospho-IκBα (Ser32, 14D4, 1:1000), phospho-JNK (Thr180/Tyr185, #9251, 1:1000), phospho-p38 (Thr180/Tyr182, 3D7, 1:1000), phospho-p105 (Ser933, 18E6, 1:1000), phospho-IKKα/β (Ser176/180, 16A6, 1:1000), phospho-TBK1 (Ser172, D52C2, 1:1000), phospho-IRF3 (Ser396, 4D4G, 1:1000), and K48-linkage-specific polyubiquitin (D9D5, 1:8000) were purchased from Cell Signaling Technology Inc. Anti-actin (C-4, 1:10,000) was from Sigma.

    Immunofluorescence:

    Article Title: Ehrlichia chaffeensis Outer Membrane Protein 1-Specific Human Antibody-Mediated Immunity Is Defined by Intracellular TRIM21-Dependent Innate Immune Activation and Extracellular Neutralization
    Article Snippet: .. Other antibodies used in this study for immunofluorescence microscopy, immunoblot, and immunoprecipitation assays include polyclonal rabbit anti-dsb antibody , anti-TRIM21 (sc-25351, sc-20960), LC3α/β (H-47; sc-292354), anti-p62/SQSTM1 (H-290; sc-25575), anti-BECN1 (E-8; sc-48341), and ULK1 (F-4; sc-390904) from Santa Cruz Biotechnology (Dallas, TX); anti-LAMP-2 (H4B4) from Developmental Studies Hybridoma Bank (University of Iowa); anti-GAPDH (10494-1-AP) from Proteintech (Rosemont, IL); and anti-K63-linkage-specific polyubiquitin (D7A11; 5621S), anti-K48-linkage-specific polyubiquitin (D9D5; 12805S), anti-NF-κB (D14E12; 8242S), anti-p62/SQSTM1 (D5E2; 8025S), anti-Atg16L1 (D6D5; 8089S), anti-ULK1 (D8H5; 8054S), and anti-LC3A/B (D3U4C, 12741S) from Cell Signaling Technology (Danvers, MA). .. Pharmacological inhibitors of the proteasome and autophagy pathways used in this study include MG-132 (474791; Calbiochem, La Jolla, CA) and 3-methyladenine (3-MA; M9281-100MG; Sigma, St. Louis, MO).

    Microscopy:

    Article Title: Ehrlichia chaffeensis Outer Membrane Protein 1-Specific Human Antibody-Mediated Immunity Is Defined by Intracellular TRIM21-Dependent Innate Immune Activation and Extracellular Neutralization
    Article Snippet: .. Other antibodies used in this study for immunofluorescence microscopy, immunoblot, and immunoprecipitation assays include polyclonal rabbit anti-dsb antibody , anti-TRIM21 (sc-25351, sc-20960), LC3α/β (H-47; sc-292354), anti-p62/SQSTM1 (H-290; sc-25575), anti-BECN1 (E-8; sc-48341), and ULK1 (F-4; sc-390904) from Santa Cruz Biotechnology (Dallas, TX); anti-LAMP-2 (H4B4) from Developmental Studies Hybridoma Bank (University of Iowa); anti-GAPDH (10494-1-AP) from Proteintech (Rosemont, IL); and anti-K63-linkage-specific polyubiquitin (D7A11; 5621S), anti-K48-linkage-specific polyubiquitin (D9D5; 12805S), anti-NF-κB (D14E12; 8242S), anti-p62/SQSTM1 (D5E2; 8025S), anti-Atg16L1 (D6D5; 8089S), anti-ULK1 (D8H5; 8054S), and anti-LC3A/B (D3U4C, 12741S) from Cell Signaling Technology (Danvers, MA). .. Pharmacological inhibitors of the proteasome and autophagy pathways used in this study include MG-132 (474791; Calbiochem, La Jolla, CA) and 3-methyladenine (3-MA; M9281-100MG; Sigma, St. Louis, MO).

    Western Blot:

    Article Title: Ehrlichia chaffeensis Outer Membrane Protein 1-Specific Human Antibody-Mediated Immunity Is Defined by Intracellular TRIM21-Dependent Innate Immune Activation and Extracellular Neutralization
    Article Snippet: .. Other antibodies used in this study for immunofluorescence microscopy, immunoblot, and immunoprecipitation assays include polyclonal rabbit anti-dsb antibody , anti-TRIM21 (sc-25351, sc-20960), LC3α/β (H-47; sc-292354), anti-p62/SQSTM1 (H-290; sc-25575), anti-BECN1 (E-8; sc-48341), and ULK1 (F-4; sc-390904) from Santa Cruz Biotechnology (Dallas, TX); anti-LAMP-2 (H4B4) from Developmental Studies Hybridoma Bank (University of Iowa); anti-GAPDH (10494-1-AP) from Proteintech (Rosemont, IL); and anti-K63-linkage-specific polyubiquitin (D7A11; 5621S), anti-K48-linkage-specific polyubiquitin (D9D5; 12805S), anti-NF-κB (D14E12; 8242S), anti-p62/SQSTM1 (D5E2; 8025S), anti-Atg16L1 (D6D5; 8089S), anti-ULK1 (D8H5; 8054S), and anti-LC3A/B (D3U4C, 12741S) from Cell Signaling Technology (Danvers, MA). .. Pharmacological inhibitors of the proteasome and autophagy pathways used in this study include MG-132 (474791; Calbiochem, La Jolla, CA) and 3-methyladenine (3-MA; M9281-100MG; Sigma, St. Louis, MO).

    Immunoprecipitation:

    Article Title: Ehrlichia chaffeensis Outer Membrane Protein 1-Specific Human Antibody-Mediated Immunity Is Defined by Intracellular TRIM21-Dependent Innate Immune Activation and Extracellular Neutralization
    Article Snippet: .. Other antibodies used in this study for immunofluorescence microscopy, immunoblot, and immunoprecipitation assays include polyclonal rabbit anti-dsb antibody , anti-TRIM21 (sc-25351, sc-20960), LC3α/β (H-47; sc-292354), anti-p62/SQSTM1 (H-290; sc-25575), anti-BECN1 (E-8; sc-48341), and ULK1 (F-4; sc-390904) from Santa Cruz Biotechnology (Dallas, TX); anti-LAMP-2 (H4B4) from Developmental Studies Hybridoma Bank (University of Iowa); anti-GAPDH (10494-1-AP) from Proteintech (Rosemont, IL); and anti-K63-linkage-specific polyubiquitin (D7A11; 5621S), anti-K48-linkage-specific polyubiquitin (D9D5; 12805S), anti-NF-κB (D14E12; 8242S), anti-p62/SQSTM1 (D5E2; 8025S), anti-Atg16L1 (D6D5; 8089S), anti-ULK1 (D8H5; 8054S), and anti-LC3A/B (D3U4C, 12741S) from Cell Signaling Technology (Danvers, MA). .. Pharmacological inhibitors of the proteasome and autophagy pathways used in this study include MG-132 (474791; Calbiochem, La Jolla, CA) and 3-methyladenine (3-MA; M9281-100MG; Sigma, St. Louis, MO).

    other:

    Article Title: Prepubertal exposure to Pb alters autophagy in the brain of aging mice: A time-series based model.
    Article Snippet: As a ubiquitous toxic heavy metal, lead (Pb) exposure is known to be implicated in the onset and development of neurodegenerative diseases which may cause more serious health hazards with age and the accumulation of Pb in the body.. Autophagy is the main degradation route for abnormal aggregated proteins and damaged cell organelles.. Here, we aimed to study the effects of adolescent Pb exposure on autophagy at different life nodes.



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    Image Search Results


    Results of Western blot analysis of p62, LC3 and mTOR protein level in the cytosol of U-138 MG cells following 24 h of treatment with CBD, celecoxib and 2,5-DMC and their combinations. Values are expressed as mean ± SEM from two independent experiments run in duplicate. Single asterisk (*) indicates the values significantly different from the DMSO-treated control (level equal to 1) with p < 0.05.

    Journal: Acta Biochimica Polonica

    Article Title: Synergistic combination of cannabidiol and celecoxib or 2,5-dimethylcelecoxib exerts oxidative stress-mediated cytotoxicity and mitigates glioblastoma invasiveness

    doi: 10.3389/abp.2025.15062

    Figure Lengend Snippet: Results of Western blot analysis of p62, LC3 and mTOR protein level in the cytosol of U-138 MG cells following 24 h of treatment with CBD, celecoxib and 2,5-DMC and their combinations. Values are expressed as mean ± SEM from two independent experiments run in duplicate. Single asterisk (*) indicates the values significantly different from the DMSO-treated control (level equal to 1) with p < 0.05.

    Article Snippet: After blocking with 10% skimmed milk for 2 h, the Immobilon P membranes were incubated overnight with the following primary antibodies against: Nrf2 (sc-365949, Santa Cruz, CA, United States), SOD-1 (sc-8637, Santa Cruz, CA, United States), CAT (sc-34285, Santa Cruz, CA, United States), p62/SQSTM1 (sc-28359, Santa Cruz, CA, United States), caspase-3 (sc-271028, Santa Cruz, CA, United States), mTOR (7C10, Cell Signalling, Danvers, MA, United States), LC3 (sc-398822, Santa Cruz, CA, United States), β-actin (sc-47778, Santa Cruz, CA, United States) and lamin A (sc-20680, Santa Cruz, CA, United States). β-actin and lamin A served as loading controls.

    Techniques: Western Blot, Control