Journal: Acta Biochimica Polonica
Article Title: Synergistic combination of cannabidiol and celecoxib or 2,5-dimethylcelecoxib exerts oxidative stress-mediated cytotoxicity and mitigates glioblastoma invasiveness
doi: 10.3389/abp.2025.15062
Figure Lengend Snippet: Results of Western blot analysis of p62, LC3 and mTOR protein level in the cytosol of U-138 MG cells following 24 h of treatment with CBD, celecoxib and 2,5-DMC and their combinations. Values are expressed as mean ± SEM from two independent experiments run in duplicate. Single asterisk (*) indicates the values significantly different from the DMSO-treated control (level equal to 1) with p < 0.05.
Article Snippet: After blocking with 10% skimmed milk for 2 h, the Immobilon P membranes were incubated overnight with the following primary antibodies against: Nrf2 (sc-365949, Santa Cruz, CA, United States), SOD-1 (sc-8637, Santa Cruz, CA, United States), CAT (sc-34285, Santa Cruz, CA, United States), p62/SQSTM1 (sc-28359, Santa Cruz, CA, United States), caspase-3 (sc-271028, Santa Cruz, CA, United States), mTOR (7C10, Cell Signalling, Danvers, MA, United States), LC3 (sc-398822, Santa Cruz, CA, United States), β-actin (sc-47778, Santa Cruz, CA, United States) and lamin A (sc-20680, Santa Cruz, CA, United States). β-actin and lamin A served as loading controls.
Techniques: Western Blot, Control